Biography Robert Hoek

1991: M.Sc. in Molecular Biology. Vrije Universiteit, Amsterdam
1995: Ph.D. in Molecular Neurobiology. Vrije Universiteit, Amsterdam
1996: Postdoctoral Research Fellow with Dr. J.D. Sedgwick, Immunopathology Research Group, Centenary Institute of Cancer Medicine and Cell Biology, Sydney, Australia
1998: Postdoctoral Research Fellow with Dr. J.D. Sedgwick, DNAX Research Institute (subsidiary of Schering-Plough, Corp.), Palo Alto, CA, USA
2001: Postdoctoral Research Fellow with Prof. Dr. H. Clevers, dept Immunology, UMC Utrecht
2005: Postdoctoral Research Fellow with Dr. J. Hamann, Dept of Experimental Immunology, AMC Amsterdam
2008: Postdoctoral Research Fellow with Dr. J. Wijnholds, Dept of Neuromedical Genetics, Netherlands Institute for Neurosciences (NIN), Royal Dutch Academy of Arts and Sciences (KNAW), Amsterdam

Publications Robert Hoek

1.         Hoek RM, Li KW, van Minnen J, Geraerts WPM. (1992) Chemical characterization of a novel peptide from the neuroendocrine light yellow cells of Lymnaea stagnalis. Mol. Brain Res. 16: 71-74
2.         Smit AB, Hoek RM, Geraerts WPM. (1993) The isolation of a cDNA encoding a neuropeptide prohormone from the light yellow cells of Lymnaea stagnalis. Cell. Mol. Neurobiol. 13: 263-270
3.         Boer HH, Montagne-Wajer C, Smith FG, Parish DC, Ramkema MD, Hoek RM, van Minnen J, Benjamin PR. (1994) Functional morphology of the light yellow cell and yellow cell (sodium influx-stimulating peptide) neuroendocrine systems of the pond snail Lymnaea stagnalis. Cell Tissue Res. 275: 361-368.
4.         Li KW, Hoek RM, Smith FG, Jimenez CR, van der Schors RC, van Veelen PA, Chen S, van der Greef J, Parish DC, Benjamin PR, Geraerts WPM. (1994) Direct peptide profiling by mass spectrometry of single identified neurons reveals complex neuropeptide-processing pattern. J. Biol. Chem. 269: 30288-30292.
5.         de Jong-Brink M, Hoek RM, Smit AB, Bergamin-Sassen MJM, Lageweg W. (1995) Schistosome parasites evoke stress responses in their snail host by a cytokine-like factor interfering with neuro-endocrine mechanisms. Neth. J. Zool. 45: 113-116.
6.         Hoek RM, Smit AB, Frings H, Vink JM, de Jong-Brink M, Geraerts WPM. (1996) A new Ig-superfamily member, molluscan defence molecule (MDM) from Lymnaea stagnalis, is down regulated during parasitosis. Eur. J. Immunol. 26: 939-944.
7.         de Jong-Brink M, Hoek RM, Lageweg W, Smit AB. (1997) Schstosome parasites induce physiological changes in their snail host by interfering with two regulatory systems, the internal defence system and the neuroendocrine system. In: Parasites and pathogens: Effects on host hormones and behavior (N. Beckage, ed.) Chapman & Hall, New York.
8.         Hoek RM, van Kesteren RE, Smit AB, Li KW, de Jong-Brink M, Geraerts WPM. (1997) Altered gene expression in the host brain caused by a trematode parasite: Neuropeptide genes are preferentially affected during parasitosis. Proc. Natl. Acad. Sci. USA 94: 14072-14076.
9.         Körner H, Cook M, Riminton DS, Lemckert FA, Hoek RM, Ledermann B, Köntgen F, Fazekas de St Groth B, Sedgwick JD. (1997) Distinct roles for lymphotoxin-a and tumor necrosis factor in organogenesis and spatial organization of lymphoid tissue. Eur. J. Immunol. 27: 2600-2609.
10.        Hoek RM, Kortekaas MC, Sedgwick JD. (1997) Allele-specific PCR analysis for detection of the gld Fas-ligand point mutation. J. Immunol. Methods 210: 109-112.
11.       Wright GJ, Puclavec M, Willis A, Hoek RM, Sedgwick JD, Brown MH, Barclay AN. (2000) Lymphoid/neuronal cell surface OX2 glycoprotein recognizes a novel receptor on macrophages implicated in the control of their function. Immunity 13: 233-242.
12.       Bakker ABH, Hoek RM, Cerwenka A, Blom B, Lucian L, McNeil T, Murray R, Phillips JH, Sedgwick JD, Lanier LL. (2000) DAP 12 deficient mice fail to develop autoimmunity due to impaired antigen priming. Immunity 13: 345-353.
13.        Hoek RM, Ruuls SR, Murphy CA, Wright G, Goddard R, Zurawski SM, Blom B, Homola ME, Streit WJ, Brown MH, Barclay AN, Sedgwick JD. (2000) Down-regulation of the macrophage lineage through interaction with OX2 (CD200). Science 290: 1768-1771.
14.        Ruuls SR, Hoek RM, Ngo V, McNeil T, Lucian L, Janatpour M, Körner H, Scheerens H, Hessel EM, Cyster J, McEvoy L, Sedgwick JD. (2001) Membrane-bound TNF supports secondary lymphoid organ structure but is subservient to secreted TNF in driving autoimmune inflammation. Immunity 15: 533-543.
15.        Wilhelm P, Riminton DS, Ritter U, Lemckert FA, Scheidig C, Hoek RM, Sedgwick JD, Körner H. (2002) Membrane lymphotoxin contributes to anti-leishmanial immunity by controlling structural integrity of lymphoid organs. Eur J. Immunol. 32: 1993-2003.
16.       Broderick C, Hoek RM, Forrester JV, Liversidge J, Sedgwick JD, Dick AD. (2002) Constitutive retinal CD200 expression regulates resident microglia and activation state of inflammatory cells during experimental autoimmune uveoretinitis. Am. J. Pathol. 161:1669-1677.
17.       Murphy CA, Hoek RM, Wiekowski MT, Lira SA, Sedgwick JD. (2002) Interactions between hemopoietically derived TNF and central nervous system-resident glial chemokines underlie initiation of autoimmune inflammation in the brain. J. Immunol. 169: 7054-7062.
18.       Hoek RM, Li KW, van Minnen J, Lodder JC, de Jong-Brink M, Smit AB, van Kesteren RE. (2005) LFRFamides: a novel family of parasitation-induced -RFamide neuropeptides that inhibit the activity of neuroendocrine cells in Lymnaea stagnalis. J Neurochem. 92:1073-1080.
19.       Kwakkenbos, M.J., Matmati, M., Madsen, O., Pouwels, W., Wang, Y., Bontrop, R.E., Heidt, P.J., Hoek, R.M., and Hamann, J. (2006). An unusual mode of concerted evolution of the EGF-TM7 receptor chimera EMR2. FASEB J. 20, 2582-2584.
20.       Matmati, M., Pouwels, W., van Bruggen, R., Jansen, M., Hoek, R.M., Verhoeven, A.J., and Hamann, J. (2007). The human EGF-TM7 receptor EMR3 is a marker for mature granulocytes. J. Leukoc. Biol. 81, 440-448.
21.       Koning, N., Bö, L., Hoek, R.M. and Huitinga, I. (2007) Down regulation of macrophage inhibitory molecules in multiple sclerosis lesions Annals Neurol. 62:504-514
22.       Rijkers, E.S.K., de Ruiter, T., Buitenhuis, M., Veninga, H., Hoek, R.M. and Meyaard, L. (2007) Ligation of CD200R by CD200 is not required for normal murine myelopoiesis. Eur J Haematol 79, 410-416.
23.       Rijkers, E.S.K, de Ruiter, T., Baridi, A., Veninga, H., Hoek, R.M., and Meyaard, L. (2007) The inhibitory CD200R is differentially expressed on human and mouse T and B lymphocytes. Mol. Immunol. 45, 1126-1135.
24.       Hoek, R.M., Veninga, H., Becker, S., van der Kaa, J., Wobus, M., Wandel, E., van der Valk, M., van Lier, R.A.W., Verbeek, J.S., Aust, G. and Hamann, J. (2007). Analysis of CD97 expression and manipulation: antibody treatment but not gene targeting curtails granulocyte migration. in press J. Immunol.
25.       Koning, N., Swaab, D.F., Huitinga I., and Hoek, R.M., Distribution of immune inhibitory molecules CD200 and CD200R in the central nervous system of MS patients and controls: indications for glia-glia interactions, Manuscript in preperation.
Other written works
a.         PhD Thesis (1995) “Parasite-induced changes in gene expression of neuroendocrine and internal defence systems of the mollusc Lymnaea stagnalis: New insights regarding survival strategies of an endo-parasite”
b.         Patent Application Hoek RM and Sedgwick JD (April 13, 1999) “Novel uses of mammalian OX2 protein and related reagents” US2002192215
c.         Patent Application Barclay AN, Brown MH, Gorman DM, Lanier LL, Wright GJ, Cherwinski H, Phillips JH, Hoek RM, Sedgwick JD “OX2 Receptor homolog” US2006084121

Personal Interest

The fascination for the complexity of organisms and how many physiological processes are regulated, lead to my specialization in Neuroscience and culminated in my PhD study on how one parasitic organism can influence physiological processes of its host through manipulation of the host’s brain. However, this latter study also kindled my ever-present interest in innate immunity, and I got the opportunity to study this in the lab of Dr Sedgwick. In the course of this work on the CD200-CD200R interaction, I got acquainted with the mouse as a model system for human diseases as well as numerous immunological techniques. This study lead to the uncovering of an important inhibitory mechanism for cells of the myeloid lineage and earned me my position at the UMC Utrecht, and later AMC Amsterdam, where I continued the work on these molecules, and expanded it towards the CD97-CD55 interaction (in the group of Dr Hamann). Currently I combine my Neurobiological and Immunological interests and skills in a project in the group of Dr Wijnholds, where we aim to isolate, by cell sorting, retinal stem cells or Müller glia cells for subretinal transplantation. These should serve the goal to develop a cell-based therapy for Crumbs (Crb) 1 related eye diseases like retinitis pigmentosa (RP) and Leber congenital amaurosis (LCA).