Biography Jeroen Dudok

2004: MSc in Neurosciences, Vrije Universiteit Amsterdam, The Netherlands

2004-2009: PhD project ‘cellular study on the outgrowth and connectivity of the brain 5-HT system’, Department of Functional Genomics, Center for Neurogenomics and Cognitive Research (CNCR), Vrije Universiteit Amsterdam, The Netherlands

2009: Postdoctoral fellow, department of Neuromedical Genetics, the Netherlands Institute for Neuroscience (NIN), Royal Netherlands Academy of Arts and Sciences (KNAW), Amsterdam, The Netherlands

Publications Jeroen Dudok

Dudok JJ, Groffen AJ, Witter MP, Voorn P, Verhage M. Chronic activation of the 5-HT₂ receptor reduces 5-HT neurite density as studied in organotypic slice cultures. Accepted for publication in Brain Research.

Groffen AJ, Brian EC, Dudok JJ, Kampmeijer J, Toonen RF, Verhage M. Ca(2+)-induced recruitment of the secretory vesicle protein DOC2B to the target membrane. J Biol Chem. 2004 May 28;279(22):23740-7.

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Personal Interests

In my PhD research project I focused on the outgrowth and connectivity of the brain serotonin system. My current project is to investigate the role of Mpp3 in retina and brain and we will use a number of approaches to elucidate the function of Mpp3. First of all, we will generate a conditional Mpp3 knockout mouse, which will allow us to study the effect of specifically inactivating Mpp3 in retina or in brain. Until we have the cKO Mpp3 mouse available, I will use shRNA mediated Mpp3 gene silencing in neurons and retina explants to investigate the effect of Mpp3 gene silencing on retinal development. Secondly, I will use dissociated neuronal cultures to investigate the expression pattern of Mpp3 in neurons, and will investigate whether absence of Mpp3 by gene silencing or in the cKO affects neuronal viability and morphology. Thirdly, I will use a number of biochemical approaches to further explore the interactions of Mpp3 with other proteins, and the complexes in which Mpp3 plays a role. Interestingly, Mpp3 affects desensitization and membrane stability of the 5-HT_2C receptor. Therefore, I will also further explore the interaction between Mpp3 and 5-HT_2C receptor and the function of this interaction. All these studies are aimed to further unravel the role of Mpp3 in the Crumbs complex and cellular polarity.

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