PERSONAL DATA
Name: Ling Shan
Nationality: China

 

Contact
Netherlands Institute for Neuroscience
Meibergdreef 47, 1105 BA Amsterdam
The Netherlands
Phone Numbers
Tel. +31.20.5665500
Email: L.shan@nin.knaw.nl

 

Education

• 2005, Bachelor (Science, College of Life Science, Hangzhou Normal University)
• 2007, Master (Science, Zhejiang University School of Medicine, China). Master program: Neurobiology

 

Postgraduate Research project
Molecular determinants of NMDA receptor assembly analyzed by fluorescence resonance energy transfer (FRET).

 

• 11/2007-present, Ph.D. student (Netherlands institute for Neuroscience, Amsterdam, The Netherlands. Supervisors: Prof. Dr. Swaab and Prof. Dr. Ai-Min Bao)

 

Ph.D. training project
The histaminergic system in neuropsychiatric disorders: a postmortem study.

Project description
The hypothalamic tuberomamillary nucleus (TMN) is the exclusive source of neuronal histamine. The histaminergic system is crucially involved in a number of basic physiological brain functions such as the sleep-wake cycle, energy and endocrine homeostasis, motor functions, attention, learning and memory, which are severely affected in neuropsychiatric diseases, such as Parkinson’s disease (PD), Huntington’s (HD), Alzheimer’s disease (AD), depression and schizophrenia.
We performed quantitative radioactive in situ hybridization of histidine decarboxylase (HDC) to study the involvement of histamine production. Moreover, the histaminergic genes expression were studied by qPCR in spcefic projection areas of Psychatiry disorders.

 

Techniques
Experiences in histology, plasmid construction, cell culturing, biochemistry techniques, imagining (including fluorescence and non-fluorescence), have a good command of data analysis and good training in neuron-anatomy.

1. Liu CQ *, Shan L *, Balesar R, Luchetti S, van Heerikhuize J, Luo JH, Swaab DF and Bao A-M. A quantitative in situ hybridization protocol for formalin-fixed paraffin-embedded archival postmortem human brain tissue. Methods. 2010 Dec;52(4):359-66. PMID: 20621191 IF: 4.5
2. van Wamelen DJ*, Shan L *, Aziz NA, Anink JJ, Bao AM, Roos RA, Swaab DF. Functional Increase of Brain Histaminergic Signaling in Huntington's Disease. Brain Pathology. 2010 Nov 25. [Epub ahead of print] PMID: 21106039 IF:4.7
3. Shan L *, Liu CQ*, van Heerikhuize J, Bao A M and Swaab D F. Neuronal histamine production remains unaltered in Parkinson’s disease despite the accumulation of Lewy bodies and Lewy neurites in the tuberomamillary nucleus Neurobiology of Aging. 2011 Mar 1. [Epub ahead of print] PMID:21371786 IF:6.6
4. Shan L, Bossers K, Bao A M and Swaab D F. Alterations in the histaminergic system in the substantia nigra and striatum of Parkinson’s patients: a postmortem study. Neurobiology of Aging 2011 Nov 25. [Epub ahead of print] PMID: 22118942 IF:6.6
5. Shan L, Hofman M., van Wamelen D J., Van Someren E J.W., Bao A M, and Swaab D F. Diurnal fluctuation in histidine decarboxylase expression, the rate limiting enzyme for histamine production, and its disorder in neurodegenerative diseases. Sleep in press. IF:5.6
6. Shan L, K Bossers, A M Bao and D F. Swaab. Alterations in the histaminergic system in Alzheimer’s disease: a postmortem study. Neurobiology of Aging in press. IF: 6.6.
7. Qiu S, Zhang XM, Cao JY, Yang W, Yan YG, Shan L, Zheng J, Luo JH.An endoplasmic reticulum retention signal located in the extracellular amino-terminal domain of the NR2A subunit of N-Methyl-D-aspartate receptors. J Biol Chem 2009 284(30): 20285-98 PMID: 19487695 IF: 5.3
8. Wilhelmus M. M., Verhaar R, Andringa G, Bol JG, Cras P, Shan L, Hoozemans JJ. M., Drukarch B. Presence of Tissue Transglutaminase in Granular Endoplasmic Reticulum is Characteristic of Melanized Neurons in Parkinson's Disease Brain. Brain pathology 2011 Mar; 21(2):130-9. Epub 2010 Aug 20. [Epub ahead of print] PMID:20731657 IF:4.7
(* indicating authors contributed equally)